免疫原
The antiserum was produced against synthesized peptide derived from human PLK3. AA range:231-280
特异性
Fnk Polyclonal Antibody detects endogenous levels of Fnk protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.,induction:Cytokine and cellular adhesion trigger FNK induction.,PTM:Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain.,tissue specificity:Transcripts are highly detected in placenta, lung, followed by skeletal mu
蛋白名称
Serine/threonine-protein kinase PLK3
其他名称
PLK3; CNK; FNK; PRK; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase
Fields
>>FoxO signaling pathway;>>C-type lectin receptor signaling pathway;>>Tuberculosis
细胞定位
Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications. .
组织表达
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
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