免疫原
The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706
特异性
DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008],
功能
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by phosphatidylserine.,function:Exhibits high phosphorylation activity for long-chain diacylglycerols.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,
蛋白名称
Diacylglycerol kinase beta
其他名称
DGKB; DAGK2; KIAA0718; Diacylglycerol kinase beta; DAG kinase beta; 90 kDa diacylglycerol kinase; Diglyceride kinase beta; DGK-beta
Fields
>>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer
细胞定位
Cell junction, synapse, postsynaptic cell membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cytoplasm . Translocation to the plasma membrane is induced by phorbol esters. .; [Isoform 2]: Cytoplasm .
组织表达
[Isoform 1]: Specifically expressed in brain but also detected in uterus (PubMed:11719522). In adult brain, expressed in the amygdala, caudate nucleus, and hippocampus (PubMed:11719522). ; [Isoform 2]: More ubiquitously expressed but at lower level compared to isoform 1.
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