免疫原
The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr360. AA range:331-380
特异性
Phospho-Bcr (Y360) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y360.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,domain:The DH domain is involved in interaction with CCPG1.,domain:The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.,function:GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.,PTM:Autophosphorylated.,similarity:Contains 1 C2 domai
蛋白名称
Breakpoint cluster region protein
其他名称
BCR; BCR1; D22S11; Breakpoint cluster region protein; Renal carcinoma antigen NY-REN-26
Fields
>>Pathways in cancer;>>Chronic myeloid leukemia
细胞定位
Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, axon . Cell junction, synapse .
组织表达
Brain,Epithelium,Platelet,Renal cell carcinoma,T-cell,
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