免疫原
The antiserum was produced against synthesized peptide derived from human DCP1A. AA range:111-160
特异性
SMIF Polyclonal Antibody detects endogenous levels of SMIF protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014],
功能
function:Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.,similarity:Belongs to the DCP1 family.,subcellular location:Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.,subunit:Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2.,tissue specificity:Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.,
蛋白名称
mRNA-decapping enzyme 1A
其他名称
DCP1A; SMIF; mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF
细胞定位
Cytoplasm, P-body . Nucleus . Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524). .
组织表达
Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
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