免疫原
The antiserum was produced against synthesized peptide derived from human GK2. AA range:281-330
特异性
GK2 Polyclonal Antibody detects endogenous levels of GK2 protein.
来源
Polyclonal, Rabbit,IgG
组成(Formulation)
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
纯化工艺(Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景(Background)
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
功能
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
其他名称
GK2; GKP2; GKTA; Glycerol kinase 2; GK 2; Glycerokinase 2; ATP:glycerol 3-phosphotransferase 2; Glycerol kinase; testis specific 2
Fields
>>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway
细胞定位
Mitochondrion outer membrane ; Single-pass type IV membrane protein . Cytoplasm . In sperm the majority of the enzyme is bound to mitochondria. .
组织表达
Testis-specific (PubMed:33536340). Expressed in the midpiece of spermatozoa (PubMed:28852571).