免疫原
The antiserum was produced against synthesized peptide derived from human PIP5K around the phosphorylation site of Ser307. AA range:273-322
特异性
Phospho-PIP5KIII (S307) Polyclonal Antibody detects endogenous levels of PIP5KIII protein only when phosphorylated at S307.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKf
功能
catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.,disease:Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD) [MIM:121850]. CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision.,function:Supports the intracellular PIP pool and to a lesser extent, the PI 4,5-P(2) pool. It generates PIP from PI and, to a lesser extent, PI 4,5-P(2) from PI 4-P. There are indications that it phosphorylates the D-5 rather than the D-4 position. Has a role in endosome-related membrane trafficking.,similarity:Contains 1 DEP domain.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 PI5K domain.,subcellular location:
蛋白名称
1-phosphatidylinositol 3-phosphate 5-kinase
其他名称
PIKFYVE; KIAA0981; PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type
Fields
>>Inositol phosphate metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phagosome;>>Regulation of actin cytoskeleton
细胞定位
Endosome membrane ; Peripheral membrane protein . Early endosome membrane ; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein . Late endosome membrane ; Peripheral membrane protein . Mainly associated with membranes of the late endocytic pathway. .
组织表达
Brain,Epithelium,PCR rescued clones,T-cell,
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