免疫原
The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. AA range:947-996
特异性
Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
IF: 1:50-200 WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act
蛋白名称
Eukaryotic translation initiation factor 2-alpha kinase 3
其他名称
EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
Fields
>>Mitophagy - animal;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis
细胞定位
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
组织表达
Ubiquitous. A high level expression is seen in secretory tissues.
联系我们
关注我们
您当前的位置： 
说明书
成功添加到购物车
