免疫原
Synthesized peptide derived from human GSDMD. AA range:1-100
特异性
The antibody detects endogenous full lenth and n-ternal fragment of gsdmd protein,
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
稀释比例
IF: 1:50-200 WB: 1:1000-3000 IHC: 1:50-300
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
功能
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).
蛋白名称
Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN
Fields
>>Neutrophil extracellular trap formation;>>NOD-like receptor signaling pathway;>>Salmonella infection
细胞定位
[Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane ; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol .
组织表达
Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
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