免疫原
The antiserum was produced against synthesized peptide derived from human AKAP12. AA range:301-350
特异性
AKAP 250 Polyclonal Antibody detects endogenous levels of AKAP 250 protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
功能
caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Antibodies to the C-terminal of gravin can be produced by patients with myasthenia gravis (MG).,domain:Polybasic regions located between residues 266 and 557 are involved in binding PKC.,function:Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).,induction:Activated by lysophosphatidylcholine (lysoPC).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 3 AKAP domains.,subcellular location:May be part of the cortical cytoskeleton.,subunit:Binds to dimeric RII-alpha regulatory subunit of PKC.,tissue specificity:Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood
蛋白名称
A-kinase anchor protein 12
其他名称
AKAP12; AKAP250; A-kinase anchor protein 12; AKAP-12; A-kinase anchor protein 250 kDa; AKAP 250; Gravin; Myasthenia gravis autoantigen
细胞定位
Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Membrane ; Lipid-anchor . May be part of the cortical cytoskeleton.
组织表达
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
联系我们
关注我们
您当前的位置： 
说明书
成功添加到购物车
