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IRP-1 (phospho Ser138) Polyclonal Antibody

说明书

BYab-03598

  • 20UL ¥460 50UL ¥945 100UL ¥1350
  • 货期: 3天左右

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宿主
反应性
应用
IHC;IF;ELISA
分子量(DA)
免疫原
The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser138. AA range:106-155
特异性
Phospho-IRP-1 (S138) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S138.
来源
Polyclonal, Rabbit,IgG
组成(Formulation)
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
纯化工艺(Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度
1 mg/ml
背景(Background)
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct
功能
catalytic activity:Citrate = isocitrate.,cofactor:Binds 1 4Fe-4S cluster per subunit.,function:Binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. This protein also expresses aconitase activity.,online information:Aconitase entry,similarity:Belongs to the aconitase/IPM isomerase family.,
基因名称(Gene Name)
ACO1
蛋白名称
Cytoplasmic aconitate hydratase
简称
IRP-1
其他名称
ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Fields
>>Citrate cycle (TCA cycle);>>Glyoxylate and dicarboxylate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids
人基因ID
48
人蛋白质序列数据库
P21399
小鼠基因ID
11428
小鼠蛋白质序列数据库
P28271
大鼠基因ID
50655
大鼠蛋白质序列数据库
Q63270
细胞定位
Cytoplasm, cytosol .
组织表达
Brain,Brain astrocytoma,Uterus,
储存(Storage)
-20°C/1 year

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IRP-1 (phospho Ser138) Polyclonal Antibody

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