免疫原
Purified recombinant human eIF2α protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of eIF2α and does not cross-react with related proteins.
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
wb dilution 1:1000 icc dilution 1:200. IF 1:50-200
纯化工艺（Immunogen）
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
背景（Background）
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],
功能
function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.,PTM:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus
其他名称
Dmel\CG6376 ;Dmel_CG6376 ;drosE2F1 ;E(Sev-CycE)3A ;E(var)3-93E ;E2-promoter binding facto ;E2F 1 ;E2F transcription factor 1 ;E2F-1 ;E2f-PA ;E2f-PB ;E2f-PC ;E2F1 ;E2f1 E2F transcription factor 1 ;E2F1_HUMAN ;Evar(3)164 ;KIAA4009 ;l(3)07172 ;l(3)j3B1 ;l(3)j3C2 ;l(3)rM729 ;mKIAA4009 ;OTTHUMP00000030661 ;PBR3 ;PRB binding protein E2F 1 ;PRB-binding protein E2F-1 ;RBAP 1 ;RBAP-1 ;RBAP1 ;RBBP-3 ;RBBP3 ;RBP 3 ;RBP3 ;Retinoblastoma-associated protein 1 ;Retinoblastoma-binding protein 3 ;Transcription factor E2F1.
Fields
>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis
细胞定位
Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .
组织表达
B cells,Brain,Fibroblast,Placenta,
联系我们
关注我们
您当前的位置： 
说明书
成功添加到购物车
