免疫原
The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110
特异性
Amylase Polyclonal Antibody detects endogenous levels of Amylase protein.
来源
Polyclonal, Rabbit,IgG
组成(Formulation)
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
纯化工艺(Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景(Background)
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
功能
catalytic activity:Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.,cofactor:Binds 1 calcium ion per subunit.,cofactor:Binds 1 chloride ion per subunit.,online information:Amylase entry,similarity:Belongs to the glycosyl hydrolase 13 family.,subunit:Monomer.,
基因名称(Gene Name)
AMY1A/AMY1B/AMY1C/AMY2A/AMY2B
蛋白名称
Alpha-amylase 1/Pancreatic alpha-amylase/Alpha-amylase 2B
其他名称
AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
细胞定位
extracellular space,extracellular exosome,
组织表达
Glandular pool- thyroid,Saliva,Thyroid,