WB;IHC;IF;ELISA

148kD

The antiserum was produced against synthesized peptide derived from human PLCG2 around the phosphorylation site of Tyr1217. AA range:1186-1235

Phospho-PLC γ2 (Y1217) Polyclonal Antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Y1217.

Polyclonal, Rabbit,IgG

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],

catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Calcium.,function:The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.,PTM:Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,

PLCG2

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

PLCG2

PLCG2; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2

>>Inositol phosphate metabolism;>>Metabolic pathways;>>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Platelet activation;>>Neutrophil extracellular trap formation;>>C-type lectin receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Thyroid hormone signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter py

5336

P16885

234779

Q8CIH5

29337

P24135

intracellular,cytosol,plasma membrane,extracellular exosome,

Lymph,Lymphoblast,Spleen,T-cell,

-20°C/1 year