免疫原
Purified recombinant human UHRF1 (N-terminus) protein fragments expressed in E.coli.
特异性
UHRF1 Monoclonal Antibody detects endogenous levels of UHRF1 protein.
组成（Formulation）
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
稀释比例
Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications.
纯化工艺（Immunogen）
Affinity purification
背景（Background）
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],
功能
developmental stage:Expressed in fetal thymus, liver and kidney.,domain:The RING finger is required for ubiquitin ligase activity.,domain:The YDG domain mediates the interaction with histone H3.,function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degrad
蛋白名称
E3 ubiquitin-protein ligase UHRF1
其他名称
UHRF1; ICBP90; NP95; RNF106; E3 ubiquitin-protein ligase UHRF1; Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106; Transcription factor ICBP90; Ubiquit
细胞定位
Nucleus . Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
组织表达
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
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