免疫原
The antiserum was produced against synthesized peptide derived from human Smad3. AA range:145-194
特异性
Smad3 Polyclonal Antibody detects endogenous levels of Smad3 protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],
功能
disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,domain:The MH2 domain is sufficient to carry protein nuclear export.,function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts wit
蛋白名称
Mothers against decapentaplegic homolog 3
其他名称
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Fields
>>FoxO signaling pathway;>>Cell cycle;>>Endocytosis;>>Cellular senescence;>>Wnt signaling pathway;>>TGF-beta signaling pathway;>>Apelin signaling pathway;>>Hippo signaling pathway;>>Adherens junction;>>Signaling pathways regulating pluripotency of stem cells;>>Th17 cell differentiation;>>AGE-RAGE signaling pathway in diabetic complications;>>Hepatitis B;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Chronic myeloid leukemia;>>Hepatocellular carcinoma;>>Gastric cancer;>>Inflammatory bowel disease;>>Diabetic cardiomyopathy
细胞定位
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity)
组织表达
Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood
联系我们
关注我们
您当前的位置： 
说明书
成功添加到购物车
