免疫原
The antiserum was produced against synthesized peptide derived from human HCFC1. AA range:131-180
特异性
HCF1 Polyclonal Antibody detects endogenous levels of HCF1 protein.
来源
Polyclonal, Rabbit,IgG
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB 1:500-2000 IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008],
功能
domain:The HCF repeat is a highly specific proteolytic cleavage signal.,domain:The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.,function:Involved in control of the cell cycle. Upon lytic infection of permissive cells, the HSV transactivator protein VP16 associates with HCFC1. Binding to HCFC1 activates VP16 for association with the octamer motif-binding protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the HSV immediate early genes. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3-K4 methyltransferase (HMT) and Sin3 histone deacetylase (HDAC) complexes (involved in the activat
其他名称
HCFC1; HCF1; HFC1; Host cell factor 1; HCF; HCF-1; C1 factor; CFF; VCAF; VP16 accessory protein
Fields
>>Herpes simplex virus 1 infection
细胞定位
Cytoplasm . Nucleus . HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm (PubMed:12235138). Non-processed HCFC1 associates with chromatin. Colocalizes with CREB3 and CANX in the ER. .
组织表达
Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
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