免疫原
Purified recombinant fragment of human AIF-M1 expressed in E. Coli.
特异性
AIF-M1 Monoclonal Antibody detects endogenous levels of AIF-M1 protein.
组成（Formulation）
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
稀释比例
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
纯化工艺（Immunogen）
Affinity purification
背景（Background）
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome
功能
catalytic activity:2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol.,cofactor:FAD.,function:Possesses significant protein thiol-disulfide oxidase activity.,function:Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). Binds to DNA in a sequence-independent manner.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 thioredoxin domain.,subcellular location:Translocated to the nucleus upon induction of apoptosis.,subunit:Interacts with XIAP.,tissue specificity:Widely expressed.,
蛋白名称
Apoptosis-inducing factor 1 mitochondrial
其他名称
AIFM1; AIF; PDCD8; Apoptosis-inducing factor 1; mitochondrial; Programmed cell death protein 8
Fields
>>Apoptosis;>>Necroptosis
细胞定位
Mitochondrion intermembrane space . Mitochondrion inner membrane. Cytoplasm . Nucleus . Cytoplasm, perinuclear region . Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969). .; [Isoform 3]: Mitochondrion intermembrane space . Mitochondrion inner membrane . Has a stronger membrane ancho
组织表达
Expressed in all tested tissues (PubMed:16644725). Detected in muscle and skin fibroblasts (at protein level) (PubMed:23217327). Expressed in osteoblasts (at protein level) (PubMed:28842795). ; [Isoform 3]: Brain specific. ; [Isoform 4]: Expressed in all tested tissues except brain. ; [Isoform 5]: Isoform 5 is frequently down-regulated in human cancers.
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